Molbank 2005, M453

http://www.mdpi.net/molbank/

 

Synthesis of Benzyl 2-(4-(8-chloro-5H-dibenzo[b,e][1,4]diazepin-11-yl)piperazin-1-yl)acetate

 

Michelle A. Camerino, Ben Capuano*, Ian T. Crosby & Edward J. Lloyd

 

Department of Medicinal Chemistry, Victorian College of Pharmacy, Monash University,

381 Royal Parade, Parkville, Victoria, 3052, Australia.

Tel. +61 3 9903 9556; Fax +61 3 9903 9582 e-mail: [email protected]

 

Received: 24 November 2005 / Accepted: 7 December 2005 / Published: 12 December 2005

 

Keywords: clozapine, amidine, benzyl ester, N-alkylation, dibenzodiazepine.

 

As part of our research programme, we have synthesized the title compound as an intermediate for the preparation of a zwitterionic analogue of the atypical antipsychotic, clozapine. The starting material, desmethylclozaine, 1 was synthesized in accordance with a previously reported literature procedure [1]. Subsequent treatment of 1 with benzyl 2-bromoacetate (2) afforded the title compound 3 in very good yield.

 

 

To a solution of desmethylclozapine (1, 503 mg, 1.61 mmol) and anhydrous triethylamine (0.451 mL, 3.23 mmol) in anhydrous 1,2-dimethoxyethane (25 mL) was added benzyl 2-bromoacetate (2, 0.287 mL, 1.81 mmol) via syringe. The reaction mixture was stirred at room temperature for 3 hours, filtered and then evaporated to dryness. The residue was treated with distilled water (10 mL) and extracted with dichloromethane (4 ´ 50 mL). The combined organic fractions were dried with anhydrous sodium sulfate, filtered, then evaporated to dryness. The resulting residue was purified using flash chromatography (silica gel 230-400 mesh, ethyl acetate:hexane, 1:1). The fractions containing product were combined and evaporated to dryness affording a yellow oil that solidified on standing. Recrystallisation from dichloromethane-hexane gave the title compound 3 as bright yellow prisms (536 mg, 72%).

 

Melting Point: 182-183˚C

 

TLC: Rf (silica; ethyl acetate:hexane, 1:1) 0.35.

 

Elemental Analysis:       Calculated for C26H25ClN4O2: C, 67.75%; H, 5.47%; N, 12.15%. Found: C, 67.62%; H, 5.51%; N, 12.17%.

 

IR (KBr, cm-1): 3320, 1728, 1600, 1558.

 

UV ((EtOH; λmax nm; log10e): 209 (4.55), 228 (4.43), 260 (4.28), 297 (4.09).

 

1H-NMR (300 MHz, CD2Cl2): d= 7.39-7.25 (m, 7 H, H1'', H3'', H2'''', H3'''', H4'''', H5'''', H6''''); 7.05-7.00 (m, 2 H, H2'', H4''); 6.87-6.81 (m, 2 H, H7'', H9''); 6.65 (d, J = 8.5 Hz, 1 H, H6''); 5.17 (s, 2 H, H1'''); 5.05 (s, 1 H, H5''); 3.46 (m, 4 H, H3', H5'); 3.33 (s, 2 H, H2); 2.67 (m, 4 H, H2', H6').

 

13C-NMR (75 MHz, CD2Cl2): d= 170.6 (C=O); 163.4 (Cq); 153.4 (Cq); 142.6 (Cq); 141.2 (Cq); 136.6 (Cq); 132.5 (CH); 130.8 (CH); 129.3 (Cq); 129.1 (CH); 128.8 (CH); 127.0 (CH); 124.0 (Cq); 123.6 (CH); 123.4 (CH); 120.7 (CH); 120.7 (CH); 120.6 (CH); 66.8 (CH2); 59.8 (CH2); 53.2 (CH2); 47.8 (CH2).

 

MS ESI (m/z, %): 463.2 (M[37Cl]H+, 32%); 461.2 (M[35Cl]H+, 100%).

 

Acknowledgment

The authors gratefully acknowledge financial support from Monash University and the assistance of Ms Anna Podloucka and Mr James Shin.

 

References:

1. Capuano, B.; Crosby, I. T.; Lloyd, E. J.; Taylor D. A. Aust. J. Chem. 2002, 55, 565.         

 

Sample Availability: Available from the author.

 

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