Guest Editor
Prof. Dr. Yoshio Umezawa
Department of Chemistry, University of Tokyo, School of Science
7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Tel. +81 3 3812 2111, ext. 4351, Fax +81 3 5802 2989
E-mail: umezawa@chem.s.u-tokyo.ac.jp; http://www.chem.s.u-tokyo.ac.jp/~analyt/member_en.html
Extended Deadline for Paper Submission: 31 March 2008
Keywords: Biosensing; Optical indicators for cellular signaling; Immunoassay; Molecular tips for chemically selective STM; DNA chips
Published Papers
Nobuaki Soh *
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University,
744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. E-mail: soh@cstf.kyushu-u.ac.jp; Phone/Fax: +81-92-802-2891
Received: 28 January 2008 / Accepted: 13 February 2008 / Published: 19 February 2008
Review: Selective Chemical Labeling of Proteins with Small Fluorescent Molecules Based on Metal-chelation Methodology
Sensors 2008, 8, 1004-1024 (PDF format, 1120 K)
Hijiri Hasegawa, Ken-ichi Taira, Koji Sode and Kazunori Ikebukuro *
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan
E-mails: sode@cc.tuat.ac.jp (Koji Sode), ikebu@cc.tuat.ac.jp (Kazunori Ikebukuro)
* Author to whom correspondence should be addressed; E-mail: ikebu@cc.tuat.ac.jp
Received: 29 January 2008 / Accepted: 15 February 2008 / Published: 19 February 2008
Review: Improvement of Aptamer Affinity by Dimerization
Sensors 2008, 8, 1090-1098 (PDF format, 683 K)
Robert M. Hoffman
AntiCancer, Inc., 7917 Ostrow Street, San Diego, CA 92111-3604, Surgery Dept., University of California, San Diego, 200 West Arbor Dr., San Diego, CA 92103-8220
Correspondence should be addressed to: Fax: 858-268-4175, E-mail: all@anticancer.com
Received: 15 January 2007 / Accepted: 19 February 2008 / Published: 22 February 2008
Review: Imaging In Mice With Fluorescent Proteins: From Macro To Subcellular
Sensors 2008, 8, 1157-1173 (PDF format, 1833 K)
Yuichi Michikawa*, Tomo Suga, Yoshimi Ohtsuka, Izumi Matsumoto, Atsuko Ishikawa, Kenichi Ishikawa, Mayumi Iwakawa, and Takashi Imai
RadGenomics Project, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, 263-8555, Japan; E-mails: tomo@nirs.go.jp, y_otsuka@nirs.go.jp, spring@nirs.go.jp, a_ishi@nirs.go.jp, k_ishi@nirs.go.jp, mayumii@nirs.go.jp, imait@nirs.go.jp
* Author to whom correspondence should be addressed; E-mail: y_michi@nirs.go.jp; Telephone: +011-81-43-206-3072 Fax: +011-81-43-206-6267
Received: 25 January 2008 / Accepted: 15 April 2008 / Published: 17 April 2008
Review: Visible Genotype Sensor Array
Sensors 2008, 8, 2722-2735 (PDF format, 422 K)
Papers
Title: "Micro-Raman Spectra and Wavelet Data Analysis for Follow-up Monitoring in Oral Pathologies."
Authors: C. Camerlingo1, F. Zenone2, G. Perna3, V. Capozzi3, N. Cirillo4, G.M. Gaeta4, M. Lepore5*
1 Consiglio Nazionale delle Ricerche, Istituto di Cibernetica “E. Caianiello”, Pozzuoli, Italy
2 Dipartimento di Scienze Fisiche, Universitą “Federico II”, Naples, Italy
3 Dipartimento di Scienze Biomediche, Universitą di Foggia, Italy
4 Dipartimento di Malattie Odontostomatologiche, Seconda Universitą di Napoli, Naples, Italy
5 Dipartimento di Medicina Sperimentale, Seconda Universitą di Napoli, Naples, Italy
* Author to whom correspondence should be addressed. E-mail : maria.lepore@unina2.it
Abstract: A wavelet multi-component decomposition algorithm has been used for data analysis of micro-Raman spectra of blood serum samples from patients affected by pemphigus vulgaris at different stages. Pemphigus is a chronic, autoimmune, blistering disease of the skin and mucous membranes with a potentially fatal outcome. Spectra were measured by means of a Raman confocal microspectrometer apparatus using the 632.8 nm line of a He-Ne laser source. A discrete wavelet transform decomposition method has been applied to the recorded Raman spectra in order to overcome problems related to low-level signals and the presence of noise and background components due to light scattering and fluorescence. A linear regression approach on the data after wavelet analysis has shown that Raman spectroscopy can provide useful information for follow-up monitoring of the investigated pathology.
Title: "Antibody fragments as probe in biosensor and microarray development"
Authors: Dirk Saerens 1,2,*, Lieven Huang 1,2, Kristien Bonroy 3 and Serge Muyldermans 1,2
1 Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium
2 Department of Molecular and Cellular Interactions, VIB, Brussels, Belgium
3 IMEC, MCP-BIO, Kapeldreef 75, B-3001 Leuven, Belgium
* Author to whom correspondence should be addressed: Dirk Saerens, E-mail: dsaerens@vub.ac.be
Abstract: Todays proteomic analyses are generating increasing numbers of biomarkers, making it essential to possess over highly specific probes able to recognize those targets. Antibodies are considered to be the first choice for such molecular recognition units due to their specificity and affinity properties, which makes them excellent probes in microarray and biosensors. However several problems, e.g. difficult immobilization, unstable behavior, loss of specificity, may arise from the use of these large molecules. Luckily, antibody/protein engineering provides a great number of novel antibody fragments suitable for biomarker analysis. Minimalization of antibodies into Fab fragments, scFv or even single-domain antibody fragments like VH, VL or VHHs is discussed. Other issues like choice of labelling tag, solid support and probe stability are of critical importance in assay design. In this respect, several specific and selective approaches to specifically orient and couple antibody fragments in a generic one-step procedure directly on a biosensor or microarray surface are discussed.
Title: "AMPEROMETRIC BIOSENSOR RESPONSES OF NOVEL CYCLOPENTADIENYLRUTHENIUM(II)THIOLATO SCHIFF BASE SELF-ASSEMBLED MONOLAYER ON GOLD"
Authors: Lawrence A. Ticha, Priscilla G.L. Baker, Hanna S. Abbo, Salam J.J. Titinchi, Emmanuel I. Iwuoha*
SensorLab, Department of Chemistry, University of Western Cape, Private Bag X17, Bellville 7535, Cape Town, South Africa.
E-mail: eiwuoha@uwc.ac.za
Abstract: A novel cyclopentadienylruthenum(II)thiolato Schiff base complex,
[Ru(SC6H4NC(H)C6H4O(CH2)2SMe)(5-C2H5)]2, was synthesized and self-assembled on gold (Au) electrode in dichloromethane. The electrochemistry of the resultant gold-self assembled monolayer (Au-SAM) electrode was studied by cyclic voltammetry (CV) and spectroelectrochemical subtractively normalised in-situ Fourier Transform Infrared spectroscopy (SINFTIR). Effective electronic communication between the Ru(II) metal centres of the complex was achieved by electrochemical demethylation of the thiolato Schiff base in 0.1 M NaOH cell within a cyclic voltammetric potential window of -200 mV to +600 mV. The SAM-modified electrode exhibited quasi-reversible electrochemistry in phosphate buffer (0.1 M, pH 7.4). This was confirmed by Randles-Sevčik analysis which gave a linear relationship between the square of the scan rate (v½) and the anodic peak current (Ip,a). The efficiency of the Au/SAM electrode in electrocatalysis was evaluated with an amperometric hydrogen peroxide biosensor constructed by electrophoretic deposition of horseradish peroxidase (HRP) on the Au/Schiff base complex at pH 7.4. The anaerobic amperometric response parameters of the biosensor for hydrogen peroxide conditions are sensitivity of 5.11 x 10-11 A/mM and detection limit of 5.26 M. Results for other environmentally important organic peroxides are also reported.
Key words: Cyclopentadienylruthenium(II)thiolato Schiff base, Self-assembled monolayer, Subtractively normalised in-situ Fourier Transform Infrared spectroscopy (SINFTIR), Spectroelectrochemistry, Amperometric peroxide biosensor.
*Corresponding Author.
Mr. Matthias Burkhalter
Assistant Editor
MDPI Center - Sensors Office
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E-mail: sensors@mdpi.org
Tel +41 61 683 7734, Fax +41 61 302 8918
http://www.mdpi.org/sensors
Submission
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MDPI - Matthias Burkhalter - 14 April 2008